Tuesday, September 1, 2009

AAS- Measurement Principle

A cathode lamp is a stable light source, which is necessary to emit the sharp characteristic spectrum of the element to be determined. A different cathode lamp is needed for each element, although there are some lamps that can be used to determine three or four different elements if the cathode contains all of them. Each time a lamp is changed, proper alignment is needed in order to get as much light as possible through the flame, where the analyte is being atomized, and into the monochromator.
The atom cell is the part with two major functions: nebulization of sample solution into a fine aerosol solution, and dissociation of the analyte elements into free gaseous ground state form. Not all the analyte goes through the flame, part of it is disposed.
As the sample passes through the flame, the beam of light passes through it into the monochromator. The monochromator isolates the specific spectrum line emitted by the light source through spectral dispersion, and focuses it upon a photomultiplier detector, whose function is to convert the light signal into an electrical signal.
The processing of electrical signal is fulfilled by a signal amplifier. The signal could be displayed for readout, or further fed into a data station for printout by the requested format.

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